| Gene | Heg1 |
| Chromosome | 16 |
| Deletion Start | 33,684,615 |
| Deletion End | 33,731,712 |
| Deletion Size | 47,098 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 33,685,222 | 33,685,244 | GATTTCGGAGTTTGGAATGCTTC |
| TUP | 33,685,253 | 33,685,280 | AGGACTTCATAAAGGACAAAGAGTGCGC |
| TUR | 33,685,306 | 33,685,327 | CCCAAGTAACCCTGCTCCTAAG |
| TDF | 33,731,011 | 33,731,029 | CCTGCAACACTGGGTCATC |
| TDP | 33,731,035 | 33,731,064 | CTCCCTAAAGATACCTACACTCTCCTGCCC |
| TDR | 33,731,094 | 33,731,115 | TTGGGAAGAGGGTCTGATTCTG |
PCR Primers
| Primer | Sequence |
| SU | AAGCCAAAGCGATACTCCAG |
| SD | TGTACATCATCAAAGGTGAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 106 bp | + | + | - |
| TDF | TDR | 105 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13548A-D3 | VGB6 | C57BL/6NTac | |
| 13548A-G4 | VGB6 | C57BL/6NTac | |
| 13548A-G7 | VGB6 | C57BL/6NTac | |
