| Gene | Wdpcp |
| Chromosome | 11 |
| Deletion Start | 21,664,381 |
| Deletion End | 21,652,253 |
| Deletion Size | 12,129 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 21,653,045 | 21,653,064 | GACACCTGCAATCAGACACC |
| TUP | 21,653,072 | 21,653,098 | TGGAGAAGAGAATTCCAGTGACAGCCA |
| TUR | 21,653,109 | 21,653,128 | TCTGCACCCTTCCAAAGTTC |
| TDF | 21,663,400 | 21,663,424 | CAGTGTTTCTCTGTGTAGCTTTCTC |
| TDP | 21,663,427 | 21,663,451 | AGAGATCCACCTGCCTCTGCCTACC |
| TDR | 21,663,458 | 21,663,480 | GGTGGTACATGCCTTTAATCCTG |
PCR Primers
| Primer | Sequence |
| SU | ACGTATGCGCCTGTACTCAG |
| SD | GAAGAACACTGCCTGCAAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 84 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13549B-A4 | VGB6 | C57BL/6NTac | |
| 13549B-A11 | VGB6 | C57BL/6NTac | |
| 13549B-G8 | VGB6 | C57BL/6NTac | |
| 13549B-H11 | VGB6 | C57BL/6NTac | |
| 13549C-H6 | VGB6 | C57BL/6NTac | |
