| Gene | Hexim1 |
| Chromosome | 11 |
| Deletion Start | 103,116,925 |
| Deletion End | 103,118,058 |
| Deletion Size | 1,134 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 103,116,925 | 103,116,943 | GCCGAGCCACTCTTGACAG |
| TUP | 103,116,948 | 103,116,976 | TCAACACCAGCCTCAAACTAGCAACTGTA |
| TUR | 103,116,991 | 103,117,013 | TCAGAGGTATGCTCTTCATGGAC |
| TDF | 103,117,683 | 103,117,701 | GGACGGCAGCGAGTTTCTG |
| TDP | 103,117,702 | 103,117,726 | CAGCGGGACTTCTCGGAGACGTACG |
| TDR | 103,117,776 | 103,117,797 | CTCCAGCTCCAGGTACTCTTTG |
PCR Primers
| Primer | Sequence |
| SU | TGCATATTTGCATACGGTGC |
| SD | TTTGGCCACCTTATAGCTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 89 bp | + | + | - |
| TDF | TDR | 115 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13551A-H10 | VGB6 | C57BL/6NTac | |
| 13551A-H2 | VGB6 | C57BL/6NTac | |
| 13551A-G3 | VGB6 | C57BL/6NTac | |
| 13551A-G9 | VGB6 | C57BL/6NTac | |
| 13551A-G5 | VGB6 | C57BL/6NTac | |
| 13551A-A6 | VGB6 | C57BL/6NTac | |
