| Gene | Higd2a |
| Chromosome | 13 |
| Deletion Start | 54,590,269 |
| Deletion End | 54,590,901 |
| Deletion Size | 633 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 54,590,334 | 54,590,352 | CTTCAGCCCCACGGTCTAC |
| TUP | 54,590,353 | 54,590,380 | TCCAACCCAGAGGGCTTTAAGGAAAAGT |
| TUR | 54,590,388 | 54,590,409 | CATTGGGTTCTCACGGGTCTTG |
| TDF | 54,590,782 | 54,590,800 | GAGCCACCGATCCCAACTC |
| TDP | 54,590,802 | 54,590,821 | TGATGCGCACCCGGATCGCT |
| TDR | 54,590,868 | 54,590,890 | TCAGGCTTGAGACTTCATAGCAG |
PCR Primers
| Primer | Sequence |
| SU | GTCTTTCCCTTAGCCTTGGG |
| SD | TAGGCATGCACTGTCAGACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 109 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13552A-A6 | VGB6 | C57BL/6NTac | |
| 13552A-H10 | VGB6 | C57BL/6NTac | |
| 13552A-A12 | VGB6 | C57BL/6NTac | |
| 13552A-B4 | VGB6 | C57BL/6NTac | |
| 13552A-B7 | VGB6 | C57BL/6NTac | |
| 13552A-G7 | VGB6 | C57BL/6NTac | |
