| Gene | Hipk4 |
| Chromosome | 7 |
| Deletion Start | 27,528,446 |
| Deletion End | 27,530,930 |
| Deletion Size | 2,485 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 27,529,344 | 27,529,361 | TGGTGTGACAGGCAGTGG |
| TUP | 27,529,364 | 27,529,388 | CCTTCTTCAGGGAGGACAAGGCTCC |
| TUR | 27,529,423 | 27,529,441 | TGGCCTCTTGCAGACTCAG |
| TDF | 27,530,500 | 27,530,519 | GACGTGGTCCAGAGGCAGAG |
| TDP | 27,530,522 | 27,530,545 | TTTGCCTGGTCTGCCAAGGCTTCC |
| TDR | 27,530,576 | 27,530,596 | AGGCCCTCACTCCCTGTGTAG |
PCR Primers
| Primer | Sequence |
| SU | AGCCAGACCTGACACCTGAC |
| SD | GCCCAATATGGGTGGTACAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 98 bp | + | + | - |
| TDF | TDR | 97 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13553A-E8 | VGB6 | C57BL/6NTac | |
| 13553A-F4 | VGB6 | C57BL/6NTac | |
| 13553A-H6 | VGB6 | C57BL/6NTac | |
| 13553A-H10 | VGB6 | C57BL/6NTac | |
| 13553A-D5 | VGB6 | C57BL/6NTac | |
