| Gene | Hoxb13 |
| Chromosome | 11 |
| Deletion Start | 96,194,446 |
| Deletion End | 96,196,260 |
| Deletion Size | 1,815 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 96,194,815 | 96,194,836 | CCCACCGAGTTTGCCTTCTATC |
| TUP | 96,194,837 | 96,194,859 | CGGGCTACCCGGGACCATACCAG |
| TUR | 96,194,875 | 96,194,894 | TGCACCACAGACACATCCAG |
| TDF | 96,196,174 | 96,196,193 | GCCGGGTCAAGGAGAAGAAG |
| TDP | 96,196,196 | 96,196,219 | TCTTGCCAAGGTCAAGACCAGCAC |
| TDR | 96,196,226 | 96,196,244 | CATCCCCTCTGGTGCTCAC |
PCR Primers
| Primer | Sequence |
| SU | AAGCGCAGAAGACTGGGAG |
| SD | CAGCTCAACTCATGAAAGCG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 80 bp | + | + | - |
| TDF | TDR | 71 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13564A-H11 | VGB6 | C57BL/6NTac | |
| 13564A-B4 | VGB6 | C57BL/6NTac | |
| 13564A-B8 | VGB6 | C57BL/6NTac | |
| 13564A-G1 | VGB6 | C57BL/6NTac | |
| 13564A-F10 | VGB6 | C57BL/6NTac | |
| 13564A-F3 | VGB6 | C57BL/6NTac | |
