| Gene | Hoxb9 |
| Chromosome | 11 |
| Deletion Start | 96,271,545 |
| Deletion End | 96,274,919 |
| Deletion Size | 3,375 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 96,271,577 | 96,271,600 | CGACTCGATCATAAGTCACGAGAG |
| TUP | 96,271,603 | 96,271,621 | AGGACGCGCCTCCAGCCAA |
| TUR | 96,271,622 | 96,271,640 | GTACTGGCCCGAAGGAAAC |
| TDF | 96,274,172 | 96,274,190 | TTCACTGGGACTGGACTGG |
| TDP | 96,274,202 | 96,274,231 | ATCTCTTGGCTATGGCTGAACAGAACTCCA |
| TDR | 96,274,260 | 96,274,280 | TGCCTTGGCTCTAGGTTTCAG |
PCR Primers
| Primer | Sequence |
| SU | CCATCCTGGATATGGAATGC |
| SD | TCTTGATGTCCTTTCTCCACG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 64 bp | + | + | - |
| TDF | TDR | 109 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13565A-A10 | VGB6 | C57BL/6NTac | |
| 13565A-B8 | VGB6 | C57BL/6NTac | |
| 13565A-C1 | VGB6 | C57BL/6NTac | |
| 13565A-E1 | VGB6 | C57BL/6NTac | |
| 13565A-H9 | VGB6 | C57BL/6NTac | |
| 13565A-B2 | VGB6 | C57BL/6NTac | |
