| Gene | Hoxc12 |
| Chromosome | 15 |
| Deletion Start | 102,936,856 |
| Deletion End | 102,938,582 |
| Deletion Size | 1,727 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 102,937,443 | 102,937,461 | GCTCTCAGCCAGCGGTAAG |
| TUP | 102,937,465 | 102,937,489 | CCCAGCCCACTTGTGATGCTTACTC |
| TUR | 102,937,551 | 102,937,571 | GCCCTAGCTCACAACACATTC |
| TDF | 102,938,351 | 102,938,368 | AGCTGGAGGGCGAGTTTC |
| TDP | 102,938,369 | 102,938,394 | TGGTCAACGAGTTCATCACACGCCAG |
| TDR | 102,938,404 | 102,938,422 | TCAAGCGGTCCGAGAGTTC |
PCR Primers
| Primer | Sequence |
| SU | GTGCAGAGGTGTGTATGTGC |
| SD | GAGTTTGGGCACATTGGTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 129 bp | + | + | - |
| TDF | TDR | 72 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13566A-B5 | VGB6 | C57BL/6NTac | |
| 13566A-B6 | VGB6 | C57BL/6NTac | |
| 13566A-G7 | VGB6 | C57BL/6NTac | |
| 13566A-F5 | VGB6 | C57BL/6NTac | |
| 13566A-A4 | VGB6 | C57BL/6NTac | |
| 13566A-C12 | VGB6 | C57BL/6NTac | |
