| Gene | Hoxc13 |
| Chromosome | 15 |
| Deletion Start | 102,921,191 |
| Deletion End | 102,927,480 |
| Deletion Size | 6,290 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 102,921,810 | 102,921,829 | CTGTTGAAGGCTACCAGCAC |
| TUP | 102,921,830 | 102,921,853 | TGGGCTCTTTCCAATGGCTGGGAC |
| TUR | 102,921,857 | 102,921,876 | TCCTTGGAGCAGTACACCTG |
| TDF | 102,927,245 | 102,927,266 | GCTGAAGGAGCTAGAGAAGGAG |
| TDP | 102,927,268 | 102,927,291 | ACGCAGCCAGCAAATTCATCACCA |
| TDR | 102,927,308 | 102,927,327 | GGTTCGTGGTGGCTGAGATG |
PCR Primers
| Primer | Sequence |
| SU | ATCTCCCAGCTCTCTCCTCC |
| SD | GTTCTTCCACAAGCTGAGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 67 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13567A-G1 | VGB6 | C57BL/6NTac | |
| 13567A-B9 | VGB6 | C57BL/6NTac | |
| 13567A-B7 | VGB6 | C57BL/6NTac | |
| 13567A-H3 | VGB6 | C57BL/6NTac | |
