| Gene | Hrc |
| Chromosome | 7 |
| Deletion Start | 45,335,430 |
| Deletion End | 45,338,930 |
| Deletion Size | 3,501 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 45,335,533 | 45,335,552 | GCCTGGGCAACTGGAACAAC |
| TUP | 45,335,553 | 45,335,576 | AATGCAGGCATCCCTGGGTCCTCA |
| TUR | 45,335,622 | 45,335,642 | GCCTCTGTCCTTCTCACCTTG |
| TDF | 45,338,357 | 45,338,374 | TGGAGCCAAGCCGAGATG |
| TDP | 45,338,375 | 45,338,401 | TGAACCGAGACTGTGGTCTGAGGTTAA |
| TDR | 45,338,430 | 45,338,451 | GATTCTGCCCACATGACCTTTG |
PCR Primers
| Primer | Sequence |
| SU | GTCCTGAGCCAGAGGAACAG |
| SD | CAGTGATCACGGGTAAAGACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 110 bp | + | + | - |
| TDF | TDR | 95 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13568A-A11 | VGB6 | C57BL/6NTac | |
| 13568A-D6 | VGB6 | C57BL/6NTac | |
| 13568A-E10 | VGB6 | C57BL/6NTac | |
| 13568A-G12 | VGB6 | C57BL/6NTac | |
| 13568A-H5 | VGB6 | C57BL/6NTac | |
| 13568A-H7 | VGB6 | C57BL/6NTac | |
