| Gene | Hs3st3b1 |
| Chromosome | 11 |
| Deletion Start | 63,921,884 |
| Deletion End | 63,889,122 |
| Deletion Size | 32,763 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 63,921,634 | 63,921,655 | TCACAGCTCCGAATGAGACATC |
| TUP | 63,921,612 | 63,921,633 | CCCTAAGATGCCCTTCCGGGCA |
| TUR | 63,921,561 | 63,921,581 | CCCAGCGCCTACTGTCTTATC |
| TDF | 63,889,754 | 63,889,775 | AACTCCAGATTGCTGCTTTGTC |
| TDP | 63,889,729 | 63,889,752 | TCGGCAGAGACTTGATGCCCAGAA |
| TDR | 63,889,684 | 63,889,703 | CAAAGTAGCTGGGCGTCTTC |
PCR Primers
| Primer | Sequence |
| SU | TGCTCTCAATCTCCTGGCTG |
| SD | CTACAGCACACTGAGGCAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 95 bp | + | + | - |
| TDF | TDR | 92 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13569A-C11 | VGB6 | C57BL/6NTac | |
| 13569A-D8 | VGB6 | C57BL/6NTac | |
| 13569A-H11 | VGB6 | C57BL/6NTac | |
| 13569A-G7 | VGB6 | C57BL/6NTac | |
| 13569A-G6 | VGB6 | C57BL/6NTac | |
| 13569A-G5 | VGB6 | C57BL/6NTac | |
