| Gene | Hs6st1 |
| Chromosome | 1 |
| Deletion Start | 36,068,661 |
| Deletion End | 36,104,312 |
| Deletion Size | 35,652 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 36,069,302 | 36,069,325 | TTCCCATCTCATCCTTCCCTACAC |
| TUP | 36,069,326 | 36,069,349 | CTGCCACTCCTGACCTCTGATCCC |
| TUR | 36,069,408 | 36,069,427 | CAGTGACCCAGGAGGAAGTC |
| TDF | 36,103,329 | 36,103,349 | GACAGGTTCCATCCCTCAGAC |
| TDP | 36,103,359 | 36,103,386 | TCTCCTCAGAAACGAGCAACGAATGTCA |
| TDR | 36,103,400 | 36,103,421 | TGGGCACATGGCATCAAGTAAG |
PCR Primers
| Primer | Sequence |
| SU | TGTACGCTAGCCGAGCCG |
| SD | CTGACCCTGACCTTGAAAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 126 bp | + | + | - |
| TDF | TDR | 93 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13570A-A2 | VGB6 | C57BL/6NTac | |
| 13570A-E9 | VGB6 | C57BL/6NTac | |
| 13570A-E2 | VGB6 | C57BL/6NTac | |
| 13570A-A8 | VGB6 | C57BL/6NTac | |
| 13570A-G9 | VGB6 | C57BL/6NTac | |
