| Gene | Hsd17b11 |
| Chromosome | 5 |
| Deletion Start | 104,021,736 |
| Deletion End | 103,990,485 |
| Deletion Size | 31,252 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 104,021,591 | 104,021,612 | GAGCTGGTCATGGGATTGGAAG |
| TUP | 104,021,559 | 104,021,588 | TGACTGCCTACGAATTTGCCAAACTTAACA |
| TUR | 104,021,511 | 104,021,529 | AGCGACGAAGCGCGGTTAC |
| TDF | 103,990,595 | 103,990,617 | TCATCTTCACACGTCCCTTTCAG |
| TDP | 103,990,567 | 103,990,592 | TCGTCCCTGAGAGGTTCCTGCAAGTC |
| TDR | 103,990,503 | 103,990,522 | GGCTGCGTCACTTGTCTTTG |
PCR Primers
| Primer | Sequence |
| SU | TGCCCTTCATCCTTTACTGC |
| SD | CACGAAATCAATCGTCATCG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 102 bp | + | + | - |
| TDF | TDR | 115 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13571A-F6 | VGB6 | C57BL/6NTac | |
| 13571A-D10 | VGB6 | C57BL/6NTac | |
| 13571A-A2 | VGB6 | C57BL/6NTac | |
| 13571A-G2 | VGB6 | C57BL/6NTac | |
