| Gene | Hspb1 |
| Chromosome | 5 |
| Deletion Start | 135,888,062 |
| Deletion End | 135,889,442 |
| Deletion Size | 1,381 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 135,888,325 | 135,888,343 | CTCGGAGATCCGACAGACG |
| TUP | 135,888,348 | 135,888,367 | ATCGCTGGCGCGTGTCCCTG |
| TUR | 135,888,437 | 135,888,456 | TCTGGGCACAAGGGAAACTC |
| TDF | 135,889,016 | 135,889,035 | GGGCTGCTTCTGACCTTCTG |
| TDP | 135,889,039 | 135,889,059 | CCACCCACAGGCAAGCACGAA |
| TDR | 135,889,060 | 135,889,079 | CCATGTTCGTCCTGCCTTTC |
PCR Primers
| Primer | Sequence |
| SU | TCCAGCTACCGGTATTACGC |
| SD | GGTTATCTCTTTTCCCACAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 132 bp | + | + | - |
| TDF | TDR | 64 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13573A-B8 | VGB6 | C57BL/6NTac | |
| 13573A-B11 | VGB6 | C57BL/6NTac | |
| 13573A-C1 | VGB6 | C57BL/6NTac | |
| 13573A-C6 | VGB6 | C57BL/6NTac | |
| 13573A-C12 | VGB6 | C57BL/6NTac | |
| 13573A-G11 | VGB6 | C57BL/6NTac | |
