| Gene | Hspb2 |
| Chromosome | 9 |
| Deletion Start | 50,752,267 |
| Deletion End | 50,751,242 |
| Deletion Size | 1,026 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 50,751,925 | 50,751,945 | TCTGATTTCGCGTCTGTCCTC |
| TUP | 50,751,899 | 50,751,924 | CCAATTCCAGCTCGCTTCCTCCATGG |
| TUR | 50,751,854 | 50,751,874 | CAGGTGGGAACTGCTGAAGAG |
| TDF | 50,751,359 | 50,751,377 | CGGCATTTGGACACGGAAG |
| TDP | 50,751,328 | 50,751,354 | TGAAGTCTACATCTCCCTGCTTCCTGC |
| TDR | 50,751,281 | 50,751,302 | AGGGCTCAACTCTGGCTATCTC |
PCR Primers
| Primer | Sequence |
| SU | GCATTGTGGATGCTATGTGG |
| SD | TATGGCGAGGAATTTGATCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 92 bp | + | + | - |
| TDF | TDR | 97 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13574A-A7 | VGB6 | C57BL/6NTac | |
| 13574A-B9 | VGB6 | C57BL/6NTac | |
| 13574A-D8 | VGB6 | C57BL/6NTac | |
| 13574A-G1 | VGB6 | C57BL/6NTac | |
| 13574A-G3 | VGB6 | C57BL/6NTac | |
| 13574A-G11 | VGB6 | C57BL/6NTac | |
