| Gene | Trmt2a |
| Chromosome | 16 |
| Deletion Start | 18,249,501 |
| Deletion End | 18,253,333 |
| Deletion Size | 3,833 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 18,249,989 | 18,250,008 | GCAGAAGCGACTGGAATGTG |
| TUP | 18,250,011 | 18,250,035 | CGGGTGCTACAGAAACTGGCCAAGT |
| TUR | 18,250,048 | 18,250,067 | CCAGCTCTGCTTGGGATGTC |
| TDF | 18,252,895 | 18,252,914 | GTCACCCACTACCTGCTCTG |
| TDP | 18,252,918 | 18,252,947 | TGCTAGATTCTAAGGTGATTCTGGCCATCC |
| TDR | 18,252,949 | 18,252,971 | GTCGCTTGATGTTCTCAGCTTTC |
PCR Primers
| Primer | Sequence |
| SU | AGACGTGCAGGAGATGTGG |
| SD | TAAATTCAAGCAAGCCCTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 77 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13575A-E7 | VGB6 | C57BL/6NTac | |
| 13575A-C1 | VGB6 | C57BL/6NTac | |
| 13575A-C8 | VGB6 | C57BL/6NTac | |
| 13575A-E2 | VGB6 | C57BL/6NTac | |
| 13575A-B3 | VGB6 | C57BL/6NTac | |
