| Gene | Ier3 |
| Chromosome | 17 |
| Deletion Start | 35,821,722 |
| Deletion End | 35,822,367 |
| Deletion Size | 646 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 35,821,942 | 35,821,960 | GAGTGGGCATCAGGAGGTC |
| TUP | 35,821,962 | 35,821,987 | CGTCGCCCTGGAACTTGTAGGTAACA |
| TUR | 35,822,002 | 35,822,023 | GAGGGAAACGTCAGATCGAAGG |
| TDF | 35,822,226 | 35,822,244 | CGAGCCTTTGAACCTGACC |
| TDP | 35,822,246 | 35,822,268 | CGGAGTCCTCGGACTATGCGCTG |
| TDR | 35,822,304 | 35,822,324 | GTGACCCATCGCGTTTAGAAG |
PCR Primers
| Primer | Sequence |
| SU | TTCACTAAGGAGGGTGGCTG |
| SD | CCCTGAGAAGCAACCAGAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 82 bp | + | + | - |
| TDF | TDR | 99 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13581A-B9 | VGB6 | C57BL/6NTac | |
