| Gene | Ikzf3 |
| Chromosome | 11 |
| Deletion Start | 98,545,747 |
| Deletion End | 98,516,856 |
| Deletion Size | 28,892 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 98,545,178 | 98,545,201 | GGCTCACGTTAAGGGTTTCATTTG |
| TUP | 98,545,139 | 98,545,168 | CGGAAGTTATAGCTTTGCTTCTCAGAAGCC |
| TUR | 98,545,054 | 98,545,075 | GAGCCACATCTTCCATCTTCAG |
| TDF | 98,517,151 | 98,517,169 | TGCGCCTCATTCACAGTTC |
| TDP | 98,517,119 | 98,517,146 | CTTTGAGAGCTTCCATATCTGCCGCTGC |
| TDR | 98,517,078 | 98,517,098 | GCATCCGGCTCTGACAAGTAG |
PCR Primers
| Primer | Sequence |
| SU | TGTTTCTCAAGTAGACGTCC |
| SD | ATTGGTGTAATGCATGTGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 148 bp | + | + | - |
| TDF | TDR | 92 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13584B-C12 | VGB6 | C57BL/6NTac | |
| 13584C-B3 | VGB6 | C57BL/6NTac | |
