| Gene | Il11 |
| Chromosome | 7 |
| Deletion Start | 4,777,983 |
| Deletion End | 4,773,556 |
| Deletion Size | 4,428 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 4,777,687 | 4,777,710 | GAGTCAGTGGGAATCTAGGAACAG |
| TUP | 4,777,660 | 4,777,683 | CAACCACGAACTCCAACAGCGCGT |
| TUR | 4,777,624 | 4,777,645 | CATCTGGGATTCTCTGCCTCAG |
| TDF | 4,773,850 | 4,773,873 | CCAAACTGCCTTTCCTATGTATCC |
| TDP | 4,773,824 | 4,773,848 | TGAATCCTGATCCTTCCGTGCTGGG |
| TDR | 4,773,793 | 4,773,812 | TGAAGCCGTATTGGGTGTTC |
PCR Primers
| Primer | Sequence |
| SU | GGAGGGTGAGTCAGGATGTG |
| SD | AATGTAGGAAGTGAGGAAGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 87 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13585A-E3 | VGB6 | C57BL/6NTac | |
| 13585A-C3 | VGB6 | C57BL/6NTac | |
| 13585A-A1 | VGB6 | C57BL/6NTac | |
| 13585A-H3 | VGB6 | C57BL/6NTac | |
| 13585A-G10 | VGB6 | C57BL/6NTac | |
