| Gene | Ilk |
| Chromosome | 7 |
| Deletion Start | 105,737,152 |
| Deletion End | 105,742,677 |
| Deletion Size | 5,526 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 105,737,457 | 105,737,478 | CGGGAACTTTGCAGCCTCTATG |
| TUP | 105,737,482 | 105,737,506 | TCATGTCAGAGGCAGACCTGAGTGC |
| TUR | 105,737,540 | 105,737,560 | TCTAGGGCGCAGAAACTCATC |
| TDF | 105,742,099 | 105,742,121 | AGGTAAAGGCATCGTATGGGAAA |
| TDP | 105,742,122 | 105,742,145 | TTGAAAAGAACCAGCTGAATAACA |
| TDR | 105,742,149 | 105,742,172 | AACCTGTCTAGTCTGTACCTCATC |
PCR Primers
| Primer | Sequence |
| SU | TCTCTGCAGCAGTTCTGAGC |
| SD | GCGAGTATGGCATCAATGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 104 bp | + | + | - |
| TDF | TDR | 74 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13586B-H10 | VGB6 | C57BL/6NTac | |
| 13586B-E3 | VGB6 | C57BL/6NTac | |
| 13586B-H3 | VGB6 | C57BL/6NTac | |
| 13586B-G8 | VGB6 | C57BL/6NTac | |
