| Gene | Inf2 |
| Chromosome | 12 |
| Deletion Start | 112,600,006 |
| Deletion End | 112,612,628 |
| Deletion Size | 12,623 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 112,600,336 | 112,600,356 | CAGCAGGGCATCGAATACATC |
| TUP | 112,600,358 | 112,600,380 | TCAGCAACCAGGGTTATGTGCGC |
| TUR | 112,600,399 | 112,600,418 | CCTAACAGAACGGCCCACAG |
| TDF | 112,611,882 | 112,611,902 | CCCAGAATCTGCACTTGACAC |
| TDP | 112,611,904 | 112,611,928 | TCCCTGGACAGGTCCTTCTCTGAGG |
| TDR | 112,611,940 | 112,611,957 | GCCAGACCCTGAGGAATC |
PCR Primers
| Primer | Sequence |
| SU | AGGCTAGCTACCTCGTGCTG |
| SD | TGGAGTCCCACTCCCTACAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 83 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13587A-A8 | VGB6 | C57BL/6NTac | |
| 13587A-B1 | VGB6 | C57BL/6NTac | |
| 13587A-B4 | VGB6 | C57BL/6NTac | |
| 13587A-C5 | VGB6 | C57BL/6NTac | |
| 13587A-C11 | VGB6 | C57BL/6NTac | |
| 13587A-F9 | VGB6 | C57BL/6NTac | |
