| Gene | Irf7 |
| Chromosome | 7 |
| Deletion Start | 141,265,633 |
| Deletion End | 141,263,127 |
| Deletion Size | 2,507 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 141,265,055 | 141,265,074 | GTGAGCAGTGCCACAAACAG |
| TUP | 141,265,023 | 141,265,047 | CCAGGGAGGTACATGGCCTCTGTCA |
| TUR | 141,264,993 | 141,265,017 | CCACAAATCAAACCATTGAAACCAC |
| TDF | 141,264,023 | 141,264,043 | AGCCTTGGGTTCCTGGATGTG |
| TDP | 141,263,999 | 141,264,022 | ACCATCATGTACAAGGGCCGCACA |
| TDR | 141,263,981 | 141,263,998 | CACCACTGCCTGTAGCAC |
PCR Primers
| Primer | Sequence |
| SU | GGACGCTGGATTAACACCTG |
| SD | ATGCTTAGAAGGTGAAGCGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 82 bp | + | + | - |
| TDF | TDR | 63 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13591A-A8 | VGB6 | C57BL/6NTac | |
| 13591A-A4 | VGB6 | C57BL/6NTac | |
| 13591A-H8 | VGB6 | C57BL/6NTac | |
| 13591A-E8 | VGB6 | C57BL/6NTac | |
| 13591A-D7 | VGB6 | C57BL/6NTac | |
