| Gene | Kcnrg |
| Chromosome | 14 |
| Deletion Start | 61,607,516 |
| Deletion End | 61,611,889 |
| Deletion Size | 4,374 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 61,607,808 | 61,607,829 | GCCAATTCCTGCTCCAATCAAG |
| TUP | 61,607,833 | 61,607,861 | TGCTGTCATGGAGGTCCATTTCCTAAACC |
| TUR | 61,607,878 | 61,607,899 | GCAAGAGCCAAACACTCTGAAG |
| TDF | 61,611,685 | 61,611,703 | GGCCAACGGAACAAATGTC |
| TDP | 61,611,705 | 61,611,734 | TGGGCCTACTGGTGGACACTTTATTAAAGG |
| TDR | 61,611,754 | 61,611,775 | TCCAGAGGCTGGTGTTCTAGTG |
PCR Primers
| Primer | Sequence |
| SU | AAGGGCGTGGTAAATGTCTG |
| SD | GAGTTCATGGCGAAAGAAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 92 bp | + | + | - |
| TDF | TDR | 91 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13596A-C10 | VGB6 | C57BL/6NTac | |
| 13596A-C8 | VGB6 | C57BL/6NTac | |
