| Gene | Krtdap |
| Chromosome | 7 |
| Deletion Start | 30,787,930 |
| Deletion End | 30,790,989 |
| Deletion Size | 3,060 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 30,788,392 | 30,788,412 | CCTGTAGTCCCTCTGCTCCTC |
| TUP | 30,788,418 | 30,788,445 | TCCTAAGGGAACAGAGGAATGCTCTTCC |
| TUR | 30,788,475 | 30,788,495 | TGCTCGAATCCAGTTGACACC |
| TDF | 30,790,175 | 30,790,194 | ATCCCTCACAAACGGCTCTG |
| TDP | 30,790,197 | 30,790,223 | TCTGTGAGCCTTGGTGTCTTCATCTGC |
| TDR | 30,790,245 | 30,790,266 | GACAGCAACCTCTTCAGGAAAC |
PCR Primers
| Primer | Sequence |
| SU | AAGAGAGGAAGGAGGGCTTG |
| SD | GTAACTAACTCGTGAACAAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 104 bp | + | + | - |
| TDF | TDR | 92 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13601A-A7 | VGB6 | C57BL/6NTac | |
| 13601A-C3 | VGB6 | C57BL/6NTac | |
| 13601A-H7 | VGB6 | C57BL/6NTac | |
| 13601A-G11 | VGB6 | C57BL/6NTac | |
| 13601A-F7 | VGB6 | C57BL/6NTac | |
| 13601A-F11 | VGB6 | C57BL/6NTac | |
