| Gene | Lamb2 |
| Chromosome | 9 |
| Deletion Start | 108,480,062 |
| Deletion End | 108,490,498 |
| Deletion Size | 10,437 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 108,480,242 | 108,480,262 | GGAGTCCTGCTGAGCTGATTG |
| TUP | 108,480,263 | 108,480,285 | ACCCACTGTCCTCAGTGCTGGCT |
| TUR | 108,480,313 | 108,480,333 | TCGAGAACAGCCAGGTACATC |
| TDF | 108,490,021 | 108,490,040 | CCGACCCTGCCTCTTACATC |
| TDP | 108,490,041 | 108,490,066 | TGCAGCAACTACGGGAACAAGTAGGT |
| TDR | 108,490,090 | 108,490,108 | AGCCTTCCGCTCTGCCAAC |
PCR Primers
| Primer | Sequence |
| SU | TGGTTCTTAGGATGAGGGTTG |
| SD | AACACGCTCTCGATTCCG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 92 bp | + | + | - |
| TDF | TDR | 88 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13602A-D12 | VGB6 | C57BL/6NTac | |
| 13602A-B5 | VGB6 | C57BL/6NTac | |
| 13602A-A7 | VGB6 | C57BL/6NTac | |
| 13602A-F11 | VGB6 | C57BL/6NTac | |
