| Gene | Leap2 |
| Chromosome | 11 |
| Deletion Start | 53,423,099 |
| Deletion End | 53,422,402 |
| Deletion Size | 698 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 53,422,915 | 53,422,933 | AGGGTGGGTGCTTCCTCAG |
| TUP | 53,422,883 | 53,422,912 | AAAGTGAGTATGCCTTTGCTCTACAGGTCA |
| TUR | 53,422,830 | 53,422,852 | GGTCATTCTCCGGGATCTCTTTG |
| TDF | 53,422,552 | 53,422,576 | CAAGAACCATTCAGATTCCTAACAC |
| TDP | 53,422,515 | 53,422,544 | CAGATGAAACAAGGAATAATGGAGGCAGCA |
| TDR | 53,422,493 | 53,422,511 | CTAAGGAGCCCTGGGACAC |
PCR Primers
| Primer | Sequence |
| SU | CCACTGTTACATTCCCAGCC |
| SD | TCAGATGGTACACACCAGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 104 bp | + | + | - |
| TDF | TDR | 84 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13605A-C7 | VGB6 | C57BL/6NTac | |
| 13605A-E5 | VGB6 | C57BL/6NTac | |
| 13605A-E8 | VGB6 | C57BL/6NTac | |
