| Gene | Lefty1 |
| Chromosome | 1 |
| Deletion Start | 180,935,113 |
| Deletion End | 180,938,076 |
| Deletion Size | 2,964 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 180,935,245 | 180,935,265 | GCTGATGTGGAAGGGATGGTC |
| TUP | 180,935,269 | 180,935,293 | CCCTCGCACGTGAGGACTCAGTATG |
| TUR | 180,935,302 | 180,935,322 | GCTGGCATGGCTGTGTTGTAG |
| TDF | 180,897,734 | 180,897,752 | TCAGCCTGCCCAACATGAG |
| TDP | 180,897,755 | 180,897,776 | TGCAGACCTGTAGCTGCGCCTC |
| TDR | 180,938,021 | 180,938,045 | GCTCTCCCAGTATAATGAGCACTTC |
PCR Primers
| Primer | Sequence |
| SU | GTCAATGGTGAAGTATATGGC |
| SD | CCAACTTGTGGCTTAGGTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 40312 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13606A-A3 | VGB6 | C57BL/6NTac | |
| 13606A-A4 | VGB6 | C57BL/6NTac | |
| 13606A-H5 | VGB6 | C57BL/6NTac | |
| 13606A-F10 | VGB6 | C57BL/6NTac | |
| 13606A-G1 | VGB6 | C57BL/6NTac | |
| 13606A-F6 | VGB6 | C57BL/6NTac | |
