| Gene | Lgals1 |
| Chromosome | 15 |
| Deletion Start | 78,926,799 |
| Deletion End | 78,930,158 |
| Deletion Size | 3,360 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 78,927,008 | 78,927,032 | GAATGTTCTGGACTTCTCGTAGTTC |
| TUP | 78,927,037 | 78,927,060 | CGCATACCTGCCTTGCAAAGTCCA |
| TUR | 78,927,084 | 78,927,103 | TTCCCACTCCCACTTCTCAG |
| TDF | 78,929,505 | 78,929,526 | GCCTTCCTGTTGCTTGAGTTTC |
| TDP | 78,929,529 | 78,929,552 | CATGGCATGTCCATAGCCAGCTGC |
| TDR | 78,929,594 | 78,929,613 | GTGGGCTTTCAGCCATGTTG |
PCR Primers
| Primer | Sequence |
| SU | GGAGAAGAATAAGAGGCGGC |
| SD | CCAGGCTATCCAGACTGCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 96 bp | + | + | - |
| TDF | TDR | 109 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13607A-B9 | VGB6 | C57BL/6NTac | |
| 13607A-C11 | VGB6 | C57BL/6NTac | |
| 13607A-H12 | VGB6 | C57BL/6NTac | |
| 13607A-G4 | VGB6 | C57BL/6NTac | |
| 13607A-G6 | VGB6 | C57BL/6NTac | |
| 13607A-G10 | VGB6 | C57BL/6NTac | |
