| Gene | Lsmd1 |
| Chromosome | 11 |
| Deletion Start | 69,395,895 |
| Deletion End | 69,396,633 |
| Deletion Size | 739 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 69,396,167 | 69,396,186 | GCCCTAGCAGGACTCTGATG |
| TUP | 69,396,190 | 69,396,210 | AACAGGAGGACTCGCCGGCCA |
| TUR | 69,396,242 | 69,396,264 | TACGGATACGCATGGTCTTGTTG |
| TDF | 69,396,282 | 69,396,301 | CTAGTCGGCTGCTTCCTGTG |
| TDP | 69,396,307 | 69,396,332 | ATCGCGACTGTAATGTCATCCTGGGC |
| TDR | 69,396,342 | 69,396,362 | ACCAGAAGGCTTGAGGAACTC |
PCR Primers
| Primer | Sequence |
| SU | AGTATCGACGAGGAGACTAG |
| SD | GCAGAGAGGGCCTTGTTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 98 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13612A-A2 | VGB6 | C57BL/6NTac | |
| 13612A-C4 | VGB6 | C57BL/6NTac | |
| 13612A-A10 | VGB6 | C57BL/6NTac | |
| 13612A-A7 | VGB6 | C57BL/6NTac | |
| 13612A-A4 | VGB6 | C57BL/6NTac | |
