| Gene | Ltb |
| Chromosome | 17 |
| Deletion Start | 35,194,525 |
| Deletion End | 35,196,162 |
| Deletion Size | 1,638 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 35,195,011 | 35,195,032 | TCTGACCTCTGGGCACTCTAAC |
| TUP | 35,195,040 | 35,195,067 | ACCCATCCAGGTTGAGAAGATCATTGGC |
| TUR | 35,195,069 | 35,195,086 | TCTGAGCCTGTGCTCCTG |
| TDF | 35,195,522 | 35,195,542 | GCAGGGATCTCGCCACTTTAG |
| TDP | 35,195,543 | 35,195,568 | TCCCTTCGGGTGATAGATAGCACCGT |
| TDR | 35,195,624 | 35,195,644 | AGGATGAGCAGGGACCTGGAG |
PCR Primers
| Primer | Sequence |
| SU | TGACAACCTTGTTTGTTGCC |
| SD | GCAACAGGTGTCCACAGTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 123 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13614A-B8 | VGB6 | C57BL/6NTac | |
| 13614A-E2 | VGB6 | C57BL/6NTac | |
| 13614A-F3 | VGB6 | C57BL/6NTac | |
| 13614A-E11 | VGB6 | C57BL/6NTac | |
