| Gene | Ly6g6e |
| Chromosome | 17 |
| Deletion Start | 35,077,158 |
| Deletion End | 35,078,347 |
| Deletion Size | 1,190 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 35,077,360 | 35,077,381 | GAGTCTGAGGCAGGAGGATCTG |
| TUP | 35,077,384 | 35,077,410 | CTCCCAGGCTAGTCTGGGCTATGTAGA |
| TUR | 35,077,412 | 35,077,434 | CCCTCTGGCCTTCTGAGACATAG |
| TDF | 35,077,802 | 35,077,822 | TTTCCTTCCTGCCTTGATCTC |
| TDP | 35,077,823 | 35,077,845 | TGCTGACCCAACAGGTCTCACCA |
| TDR | 35,077,906 | 35,077,924 | CGACACTCCCTGGGAACAG |
PCR Primers
| Primer | Sequence |
| SU | CTAGAACCGGGAACAGCTTG |
| SD | GCCCTCTGTTCTTGAGACCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 123 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13615A-A2 | VGB6 | C57BL/6NTac | |
| 13615A-B9 | VGB6 | C57BL/6NTac | |
| 13615A-G11 | VGB6 | C57BL/6NTac | |
| 13615A-F8 | VGB6 | C57BL/6NTac | |
| 13615A-G2 | VGB6 | C57BL/6NTac | |
| 13615A-E12 | VGB6 | C57BL/6NTac | |
