| Gene | Macrod1 |
| Chromosome | 19 |
| Deletion Start | 7,056,894 |
| Deletion End | 7,066,196 |
| Deletion Size | 9,303 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 7,057,758 | 7,057,779 | AGTCGCTAACTGGAATGCAAAG |
| TUP | 7,057,788 | 7,057,812 | CCCAAATAGCCCTGAGTGGCAGTCC |
| TUR | 7,057,825 | 7,057,843 | GAAGCACTGGCGCTGAAAG |
| TDF | 7,065,732 | 7,065,752 | GCAGTCCCATGACAGCATTAG |
| TDP | 7,065,754 | 7,065,777 | AAGCCAGCTCAAGCGAGGCCACAT |
| TDR | 7,065,818 | 7,065,838 | AAGTGCGCCACAGCATCAAAC |
PCR Primers
| Primer | Sequence |
| SU | GGCGGAAAGAACTAGAAGCC |
| SD | TGTGGCTTCATTCCAGACAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 86 bp | + | + | - |
| TDF | TDR | 107 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13617A-A7 | VGB6 | C57BL/6NTac | |
| 13617A-B11 | VGB6 | C57BL/6NTac | |
| 13617A-C5 | VGB6 | C57BL/6NTac | |
| 13617A-C6 | VGB6 | C57BL/6NTac | |
| 13617A-H8 | VGB6 | C57BL/6NTac | |
| 13617A-G5 | VGB6 | C57BL/6NTac | |
