| Gene | Madcam1 |
| Chromosome | 10 |
| Deletion Start | 79,664,610 |
| Deletion End | 79,668,443 |
| Deletion Size | 3,834 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 79,664,962 | 79,664,981 | GGCACATCCCTCCAGATCAC |
| TUP | 79,664,985 | 79,665,009 | CAGCATGTCCTGTGACGAGGGTGTA |
| TUR | 79,665,029 | 79,665,047 | CCCAAGCTGGTGTCCAGAC |
| TDF | 79,667,585 | 79,667,606 | TCAGGAGGCAGAGACAAGAAAC |
| TDP | 79,667,610 | 79,667,636 | TAGCCACTTGTAACTCCAGGACCCTGG |
| TDR | 79,667,640 | 79,667,662 | GGAAGTCAGAGGAGGATGTCAAG |
PCR Primers
| Primer | Sequence |
| SU | ACCTGTGGAAGTGAAGGCAC |
| SD | TATTCCCTTGTCTGGCATCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 86 bp | + | + | - |
| TDF | TDR | 78 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13618A-D6 | VGB6 | C57BL/6NTac | |
| 13618A-F7 | VGB6 | C57BL/6NTac | |
| 13618A-F10 | VGB6 | C57BL/6NTac | |
| 13618A-H3 | VGB6 | C57BL/6NTac | |
| 13618A-D11 | VGB6 | C57BL/6NTac | |
