| Gene | Maff |
| Chromosome | 15 |
| Deletion Start | 79,357,001 |
| Deletion End | 79,357,891 |
| Deletion Size | 891 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 79,357,232 | 79,357,254 | GGTTAGTTTGAGCCTTGGGTGAC |
| TUP | 79,357,257 | 79,357,281 | CCTGTAGGCTGATGCTGGTCCTGCT |
| TUR | 79,357,299 | 79,357,319 | TCGGCGGTTACTGGACTTGAG |
| TDF | 79,357,551 | 79,357,568 | GCCGCACACTCAAGAACC |
| TDP | 79,357,570 | 79,357,588 | CGGCTACGCGGCCAGCTGC |
| TDR | 79,357,624 | 79,357,642 | CAGCTCGGACTTCTGCTTC |
PCR Primers
| Primer | Sequence |
| SU | TGACTGACCTTGGTGTGGAG |
| SD | CTTCCCAGCAGGACAGTCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 88 bp | + | + | - |
| TDF | TDR | 92 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13619B-A12 | VGB6 | C57BL/6NTac | |
| 13619B-D11 | VGB6 | C57BL/6NTac | |
| 13619A-D5 | VGB6 | C57BL/6NTac | |
| 13619B-C8 | VGB6 | C57BL/6NTac | |
