| Gene | Maml1 |
| Chromosome | 11 |
| Deletion Start | 50,266,644 |
| Deletion End | 50,257,747 |
| Deletion Size | 8,898 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 50,265,910 | 50,265,929 | TGATGCCCGGTGTGAACAAG |
| TUP | 50,265,888 | 50,265,909 | AGTTCTCCTCGGCCAGGAGGCC |
| TUR | 50,265,859 | 50,265,879 | CAGGTTGGAGTGGCTAGGTTG |
| TDF | 50,258,409 | 50,258,429 | CACAAGTCCCAAGGGTGTCAG |
| TDP | 50,258,386 | 50,258,408 | CAGCCATGGGAAGCCAGAATGCC |
| TDR | 50,258,350 | 50,258,369 | GCTGCTCAGGTTTGGCATTC |
PCR Primers
| Primer | Sequence |
| SU | GTGAGGGAGCAATCAAGGTC |
| SD | AGAAGGCCTCAACTCCCAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 71 bp | + | + | - |
| TDF | TDR | 80 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13620A-A10 | VGB6 | C57BL/6NTac | |
| 13620A-B7 | VGB6 | C57BL/6NTac | |
| 13620A-C10 | VGB6 | C57BL/6NTac | |
| 13620A-E3 | VGB6 | C57BL/6NTac | |
| 13620A-F7 | VGB6 | C57BL/6NTac | |
