| Gene | Med11 |
| Chromosome | 11 |
| Deletion Start | 70,451,982 |
| Deletion End | 70,453,330 |
| Deletion Size | 1,349 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 70,452,270 | 70,452,290 | GCAGCCTTCACCACTTCTGTG |
| TUP | 70,452,294 | 70,452,317 | CACGTGGAGGCAGAGCTATCCGCT |
| TUR | 70,452,318 | 70,452,339 | CCTGAGTGAGGTAGCGGATTTG |
| TDF | 70,453,122 | 70,453,142 | TGAGGGCTCCAGCTACTCTTC |
| TDP | 70,453,143 | 70,453,172 | CAGGAAGGACTGTCAAATGGCTCTAAAGCG |
| TDR | 70,453,199 | 70,453,217 | CAGGTTCTCGCCACATCAC |
PCR Primers
| Primer | Sequence |
| SU | AGTCAGACTAGACCAGGTAG |
| SD | TTTGTTTCTTCCAATGAGGAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 70 bp | + | + | - |
| TDF | TDR | 96 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13624A-A1 | VGB6 | C57BL/6NTac | |
| 13624A-A9 | VGB6 | C57BL/6NTac | |
| 13624A-A12 | VGB6 | C57BL/6NTac | |
| 13624A-B4 | VGB6 | C57BL/6NTac | |
| 13624A-B10 | VGB6 | C57BL/6NTac | |
| 13624A-F4 | VGB6 | C57BL/6NTac | |
