| Gene | Mfap3 |
| Chromosome | 11 |
| Deletion Start | 57,527,924 |
| Deletion End | 57,530,241 |
| Deletion Size | 2,318 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 57,528,025 | 57,528,046 | CATCACTGCCTGTCCATCTTAC |
| TUP | 57,528,047 | 57,528,068 | TGGTGGTCACTCTTGTGCCAGC |
| TUR | 57,528,082 | 57,528,101 | CCCAGTGGAGCCACATCTTC |
| TDF | 57,530,100 | 57,530,121 | GCAGGAGATAGCAGTACAGGTG |
| TDP | 57,530,122 | 57,530,147 | TCTGTCCACCTTCAGTCAGAGACCAA |
| TDR | 57,530,177 | 57,530,194 | TGGGTGGGCTGAAATGAC |
PCR Primers
| Primer | Sequence |
| SU | TCATCCTTCTCCTCAGTGGG |
| SD | ACCAGCACATGCTCACTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 77 bp | + | + | - |
| TDF | TDR | 95 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13629C-C4 | VGB6 | C57BL/6NTac | |
| 13629C-D9 | VGB6 | C57BL/6NTac | |
| 13629C-E11 | VGB6 | C57BL/6NTac | |
| 13629C-G9 | VGB6 | C57BL/6NTac | |
| 13629C-H9 | VGB6 | C57BL/6NTac | |
