| Gene | Mfsd10 |
| Chromosome | 5 |
| Deletion Start | 34,636,764 |
| Deletion End | 34,633,832 |
| Deletion Size | 2,933 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 34,636,077 | 34,636,095 | CAGTTCTTCTCCGCACCAC |
| TUP | 34,636,050 | 34,636,075 | CACAGGAGCTGCCTCTGATTACCTGG |
| TUR | 34,636,019 | 34,636,038 | ACCAGGGACAACATCATCAC |
| TDF | 34,634,436 | 34,634,455 | CGTTGGGCTTGGGACTGATG |
| TDP | 34,634,408 | 34,634,432 | TACTCCTTTGGTGAGTGGGCTCTGC |
| TDR | 34,634,373 | 34,634,392 | CTAGGAGAGGCACGAGGATG |
PCR Primers
| Primer | Sequence |
| SU | AGGTGGGAAGCAAAGGTTTC |
| SD | GGGTTGTTAGAAAGTGGTTCTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 77 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13630A-A7 | VGB6 | C57BL/6NTac | |
| 13630A-C3 | VGB6 | C57BL/6NTac | |
| 13630A-F11 | VGB6 | C57BL/6NTac | |
| 13630A-E3 | VGB6 | C57BL/6NTac | |
| 13630A-E1 | VGB6 | C57BL/6NTac | |
