| Gene | Mfsd3 |
| Chromosome | 15 |
| Deletion Start | 76,701,672 |
| Deletion End | 76,703,491 |
| Deletion Size | 1,820 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 76,702,217 | 76,702,234 | TGCACCAGCCTTGGGAAG |
| TUP | 76,702,240 | 76,702,261 | CATGGCCTCAGGCCTCAGAGCA |
| TUR | 76,702,287 | 76,702,306 | GGCACAGCCAGCAAATCTTG |
| TDF | 76,703,122 | 76,703,140 | TCACCACTGCCACATTCAC |
| TDP | 76,703,143 | 76,703,168 | TGATGATGCACTGTAGCCAGCTAGCA |
| TDR | 76,703,181 | 76,703,204 | ATCCCTACCCTTCCCTCTTACCTG |
PCR Primers
| Primer | Sequence |
| SU | GCAGATGATTTGGGTCTGTG |
| SD | CTAGCGAGTCCATGCTACCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 90 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13631A-E8 | VGB6 | C57BL/6NTac | |
| 13631A-A10 | VGB6 | C57BL/6NTac | |
| 13631A-B3 | VGB6 | C57BL/6NTac | |
| 13631A-C5 | VGB6 | C57BL/6NTac | |
| 13631A-D10 | VGB6 | C57BL/6NTac | |
| 13631A-E6 | VGB6 | C57BL/6NTac | |
