| Gene | Mkl2 |
| Chromosome | 16 |
| Deletion Start | 13,358,642 |
| Deletion End | 13,385,780 |
| Deletion Size | 27,139 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 13,359,513 | 13,359,533 | TCACTCCCTCAGCTCTGTCTG |
| TUP | 13,359,534 | 13,359,555 | CAGACCCTTCCTGGCCTGCTCA |
| TUR | 13,359,586 | 13,359,606 | CAGGGCAACAGGGTTCCTTAC |
| TDF | 13,385,039 | 13,385,063 | AGCAGGGAGGTGTGAGTGTAGTTAG |
| TDP | 13,385,065 | 13,385,091 | TGTGCTGTTCTTTGAAATGGAGGCTGA |
| TDR | 13,385,118 | 13,385,140 | TGACCTGGAAACAGAGGAGAAAG |
PCR Primers
| Primer | Sequence |
| SU | TGCTTGACTCAGTGCTCGTC |
| SD | GTGTGTGTATATCTATCAGAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 94 bp | + | + | - |
| TDF | TDR | 102 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13633A-G1 | VGB6 | C57BL/6NTac | |
| 13633A-C10 | VGB6 | C57BL/6NTac | |
| 13633A-F1 | VGB6 | C57BL/6NTac | |
| 13633A-F9 | VGB6 | C57BL/6NTac | |
| 13633A-F8 | VGB6 | C57BL/6NTac | |
| 13633A-C2 | VGB6 | C57BL/6NTac | |
