| Gene | Camk2g |
| Chromosome | 14 |
| Deletion Start | 20,793,962 |
| Deletion End | 20,764,151 |
| Deletion Size | 29,812 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 20,793,096 | 20,793,116 | GCTGTCACAATCAGAGCTGTC |
| TUP | 20,793,068 | 20,793,093 | TCCTCTGTTTCCCTGCTGTCAGTTGA |
| TUR | 20,793,024 | 20,793,042 | CGCTCAAGATCCGCTCAAC |
| TDF | 20,764,266 | 20,764,284 | TGTTCCCGCTCCTCAGTTC |
| TDP | 20,764,237 | 20,764,262 | TCACCAGAATGGGACACAGTCACTCC |
| TDR | 20,764,186 | 20,764,208 | CGCTTTGCAGGGTTTATGGTCAG |
PCR Primers
| Primer | Sequence |
| SU | AGGAGGTGTCTTCCAGTTCG |
| SD | CTTCCAATCCAGCAACAGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 93 bp | + | + | - |
| TDF | TDR | 99 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13634A-G2 | VGB6 | C57BL/6NTac | |
| 13634A-A7 | VGB6 | C57BL/6NTac | |
| 13634A-F6 | VGB6 | C57BL/6NTac | |
| 13634A-H7 | VGB6 | C57BL/6NTac | |
