| Gene | Wnt5a |
| Chromosome | 14 |
| Deletion Start | 28,522,955 |
| Deletion End | 28,506,112 |
| Deletion Size | 16,844 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 28,506,525 | 28,506,545 | GCCGGATGAGGACTGTGTTAG |
| TUP | 28,506,549 | 28,506,577 | AAACGGCTGCGAGACTAAGGAAACTAGGG |
| TUR | 28,506,580 | 28,506,599 | GCGTTGCTCTAGGGTAGGTG |
| TDF | 28,522,847 | 28,522,867 | GCAGACCGAACGCTGTCATTG |
| TDP | 28,522,872 | 28,522,899 | TTTCACTGGTGCTGCTATGTCAAATGCA |
| TDR | 28,522,934 | 28,522,954 | GGGCAGGCACACCACTATTTG |
PCR Primers
| Primer | Sequence |
| SU | TCGCTGATGGACGTTAGAGA |
| SD | AAGGACTCTCTGTTTGAGAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 108 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13635A-G10 | VGB6 | C57BL/6NTac | |
| 13635B-D12 | VGB6 | C57BL/6NTac | |
| 13635B-F2 | VGB6 | C57BL/6NTac | |
| 13635C-E11 | VGB6 | C57BL/6NTac | |
| 13635C-F3 | VGB6 | C57BL/6NTac | |
