| Gene | Mlx |
| Chromosome | 11 |
| Deletion Start | 101,087,329 |
| Deletion End | 101,091,211 |
| Deletion Size | 3,883 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 101,087,755 | 101,087,777 | TCGAGTATGCCTACAGTGACAAC |
| TUP | 101,087,778 | 101,087,802 | AGCCTGGATCCTGGTGAGTGCCTTC |
| TUR | 101,087,831 | 101,087,853 | AAGAATCTACAGAGCCGAACCAG |
| TDF | 101,091,125 | 101,091,147 | TGATCGGTGTCCTTCATCAGTTG |
| TDP | 101,091,154 | 101,091,180 | CAACTCTACTGATTGCCCATGGAAGCC |
| TDR | 101,091,183 | 101,091,202 | GCCTCTTGTTGGCTGCTCTG |
PCR Primers
| Primer | Sequence |
| SU | GAAATTCTTGACCCAGCAGC |
| SD | GAGACCAAGCAGAGGCAAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 99 bp | + | + | - |
| TDF | TDR | 78 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13636A-D6 | VGB6 | C57BL/6NTac | |
| 13636A-B1 | VGB6 | C57BL/6NTac | |
| 13636A-B3 | VGB6 | C57BL/6NTac | |
| 13636A-B2 | VGB6 | C57BL/6NTac | |
| 13636A-B6 | VGB6 | C57BL/6NTac | |
