| Gene | Mmp23 |
| Chromosome | 4 |
| Deletion Start | 155,653,177 |
| Deletion End | 155,650,620 |
| Deletion Size | 2,558 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 155,652,658 | 155,652,678 | TCTATCCCGTTGCCTAAAGTC |
| TUP | 155,652,632 | 155,652,657 | TGCTTCTGATAACAACTCTCGGGCAC |
| TUR | 155,652,592 | 155,652,613 | AGGGAGAAGTGAATGTGAGGAG |
| TDF | 155,650,743 | 155,650,761 | GGCACCAGCGTGTTCTCAG |
| TDP | 155,650,717 | 155,650,741 | ACCTACTCCTGGAGGGTCCGAGTGA |
| TDR | 155,650,666 | 155,650,686 | GTGCTTTATCTGCTGCCTTCC |
PCR Primers
| Primer | Sequence |
| SU | TGTCATTCAACTCCGACCAG |
| SD | GCCTAGACTTGGGTGAGCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 87 bp | + | + | - |
| TDF | TDR | 96 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13637A-B1 | VGB6 | C57BL/6NTac | |
| 13637A-E9 | VGB6 | C57BL/6NTac | |
| 13637A-F10 | VGB6 | C57BL/6NTac | |
| 13637A-H1 | VGB6 | C57BL/6NTac | |
| 13637A-H3 | VGB6 | C57BL/6NTac | |
