| Gene | Mn1 |
| Chromosome | 5 |
| Deletion Start | 111,418,169 |
| Deletion End | 111,454,800 |
| Deletion Size | 36,632 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 111,418,842 | 111,418,864 | GAGCCGTAGATTCCCTGGAATAC |
| TUP | 111,418,867 | 111,418,886 | TTACCCGAGCGAGCCGCCCT |
| TUR | 111,418,912 | 111,418,931 | GCTGCCCTTCAGAGTCAGAG |
| TDF | 111,453,859 | 111,453,878 | TGTTCCTTTCTGGGCCACAG |
| TDP | 111,453,879 | 111,453,903 | ATGCCTCGTCTGTGAAGTGCAGGAC |
| TDR | 111,453,934 | 111,453,954 | GCTGGCAAGGAGAAGCCTATG |
PCR Primers
| Primer | Sequence |
| SU | TTTGTCTCAGCTCGTCGGC |
| SD | AGCCTTCTAGAACACACGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 90 bp | + | + | - |
| TDF | TDR | 96 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13638A-D2 | VGB6 | C57BL/6NTac | |
| 13638A-D3 | VGB6 | C57BL/6NTac | |
| 13638A-D4 | VGB6 | C57BL/6NTac | |
| 13638A-D6 | VGB6 | C57BL/6NTac | |
| 13638A-D8 | VGB6 | C57BL/6NTac | |
