| Gene | Mob3a |
| Chromosome | 10 |
| Deletion Start | 80,691,486 |
| Deletion End | 80,687,111 |
| Deletion Size | 4,376 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 80,690,731 | 80,690,751 | GCCCAGGGTATAGCGATCTTC |
| TUP | 80,690,703 | 80,690,728 | AGTTCAGTGGTTCTCCGCCTTCCTAA |
| TUR | 80,690,651 | 80,690,670 | TGAGGCCAGCACAGTATGAG |
| TDF | 80,687,242 | 80,687,264 | ATGCCCACTGTTCAGCGTCTAAC |
| TDP | 80,687,211 | 80,687,240 | TGTCTTGGGTTGTATGAGGTCTTTGCTACC |
| TDR | 80,687,153 | 80,687,176 | CATCCGTGATGTCATCTCTTTCTG |
PCR Primers
| Primer | Sequence |
| SU | CCTACGCTTACACAGGTCCC |
| SD | AGGTATGCTGGCTGCCTATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 101 bp | + | + | - |
| TDF | TDR | 112 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13639A-A8 | VGB6 | C57BL/6NTac | |
| 13639A-C3 | VGB6 | C57BL/6NTac | |
| 13639A-C5 | VGB6 | C57BL/6NTac | |
| 13639A-E10 | VGB6 | C57BL/6NTac | |
| 13639A-G3 | VGB6 | C57BL/6NTac | |
| 13639A-D3 | VGB6 | C57BL/6NTac | |
