| Gene | Mrpl11 |
| Chromosome | 19 |
| Deletion Start | 4,962,396 |
| Deletion End | 4,964,833 |
| Deletion Size | 2,438 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 4,962,831 | 4,962,851 | GAAAGCCTTCAGCTCCCATAC |
| TUP | 4,962,859 | 4,962,887 | TGTGTATGCGTTCCTTATAGCGAGGTGTC |
| TUR | 4,962,909 | 4,962,931 | TCCTTTGTCTTCTCGTTGAACTC |
| TDF | 4,964,296 | 4,964,316 | ACAGGAGCAGGGACAGTCATC |
| TDP | 4,964,317 | 4,964,339 | ACTGGCCGCTTGCTGCTGGTGTC |
| TDR | 4,964,345 | 4,964,364 | TGACGCCAGTCTTGCTGTAG |
PCR Primers
| Primer | Sequence |
| SU | TTGGCTGTCTAAGGTGATGC |
| SD | AGCACCATGAATCAGAAGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 101 bp | + | + | - |
| TDF | TDR | 69 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13641A-D4 | VGB6 | C57BL/6NTac | |
| 13641A-G6 | VGB6 | C57BL/6NTac | |
| 13641A-F11 | VGB6 | C57BL/6NTac | |
| 13641A-G3 | VGB6 | C57BL/6NTac | |
| 13641A-B8 | VGB6 | C57BL/6NTac | |
