| Gene | Mrpl28 |
| Chromosome | 17 |
| Deletion Start | 26,123,565 |
| Deletion End | 26,126,463 |
| Deletion Size | 2,899 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 26,123,825 | 26,123,849 | CTACAGATACGCCAACAACGACAAG |
| TUP | 26,123,855 | 26,123,876 | TGCGGACCATCTCGGCTGTTCT |
| TUR | 26,123,882 | 26,123,900 | CAGCGCCTGCTCAGCAAAG |
| TDF | 26,125,995 | 26,126,019 | AGGATGTTCCATTTGTGTCTGTTTC |
| TDP | 26,126,025 | 26,126,048 | ATAGTCAAGAGCCTGTGGCGGCCA |
| TDR | 26,126,050 | 26,126,070 | CCCTTGGCTTCCCTGTAACTG |
PCR Primers
| Primer | Sequence |
| SU | AGGGTAGCCTTTCCCAACAG |
| SD | AATGATCCGGACAAGAATGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13642A-D8 | VGB6 | C57BL/6NTac | |
| 13642B-E9 | VGB6 | C57BL/6NTac | |
| 13642B-F2 | VGB6 | C57BL/6NTac | |
