| Gene | Mtmr2 |
| Chromosome | 9 |
| Deletion Start | 13,749,120 |
| Deletion End | 13,785,923 |
| Deletion Size | 36,804 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 13,749,803 | 13,749,824 | GACCAGCCCATTACCAGACAAG |
| TUP | 13,749,829 | 13,749,854 | TTTAGAGGACACTGAGACAAGCGTGC |
| TUR | 13,749,884 | 13,749,906 | AAGCTGAGAAGCTGACTCTGAAG |
| TDF | 13,785,055 | 13,785,077 | GCATAGTTTAGCTCAGTGTCCTC |
| TDP | 13,785,078 | 13,785,104 | TGTGCTACAGGATCTTACCCAGAGCAC |
| TDR | 13,785,154 | 13,785,175 | TGAGAGTCACTGGGAACAAGAG |
PCR Primers
| Primer | Sequence |
| SU | GGATCAGTCTGCTTAGCTTC |
| SD | TTAGCTAGCCAACACAGTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 104 bp | + | + | - |
| TDF | TDR | 121 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13653A-C4 | VGB6 | C57BL/6NTac | |
| 13653B-G1 | VGB6 | C57BL/6NTac | |
