| Gene | Mtmr9 |
| Chromosome | 14 |
| Deletion Start | 63,543,636 |
| Deletion End | 63,524,052 |
| Deletion Size | 19,585 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 63,543,589 | 63,543,609 | CGGGTAGACAATGTGGTGCTG |
| TUP | 63,543,565 | 63,543,587 | ACCGGCCTTTCTACACCGCCGTG |
| TUR | 63,543,516 | 63,543,534 | GCCGGGAAGACAGGATCAG |
| TDF | 63,524,808 | 63,524,827 | AACAGCCTGTGCCTCTGATG |
| TDP | 63,524,784 | 63,524,806 | CGCGGCCACCATCACTCTCACAG |
| TDR | 63,524,752 | 63,524,771 | GGAGATTGCCTGCCTGAGTG |
PCR Primers
| Primer | Sequence |
| SU | TCCGGTTTCTATGTCTTCCG |
| SD | ACTGGGAGTGGAGGATAGGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 94 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13654A-E7 | VGB6 | C57BL/6NTac | |
| 13654A-E1 | VGB6 | C57BL/6NTac | |
